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KMID : 0613820040140061018
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Journal of Life Science
2004 Volume.14 No. 6 p.1018 ~ p.1022
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In Vitro Culture and Transformation by Agroinfiltration of Lisianthus (Eustoma russellianus) Pollen
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Park Hee-Sung
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Abstract
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Optimized conditions for Agrobacterium-mediated lisianthus pollen transformation were adjusted using various factors such as temperature, pH and sucrose concentration. Pollen tube growth was successfully achieved in a medium (pollen germination medium; PGM) containing 7-15% sucrose with pH in the range of 5.5-7.0 at temperature of 20-27oC. Lisianthus pollen was vacuum-infiltrated with Agrobacterium cell suspension for 20 min, and transformed pollen was confirmed by GUS histochemistry and Southern hybridization following RT-PCR. Transgenic pollen system may be utilized for establishing an area of plant transient expression systems based on the convenient pollen transformation procedure presented in here.
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KEYWORD
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Lisianthus, pollen, pollen germination medium, transformation, GUS gene
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